I am a staff scientist in the Life Sciences Division at the Lawrence Berkeley National Laboratory (since 2004). Furthermore as the Director of Physical Analysis of the Joint BioEnergy Institute (since 2007), Program Lead for Imaging at the Energy Biosciences Institute (2007-2012) and more recently Divisional Program Lead for Mesoscale BioImaging Technologies (since 2014) I have experience in assembling and overseeing a team of experts from various imaging disciplines, ranging from optical microscopy, vibrational spectroscopy imaging and other chemical imaging approaches such as mass spectrometry imaging, to various 3D electron microscopy imaging and integrated quantitative image analysis, as is evident from numerous publications as well as multiple roles in bioimaging and bioimage informatics meeting organization, steering committee membership, reviewer assignments, and integrated bioimaging book authorship.
The goal of the research in my lab is to determine the macromolecular and cellular multiprotein organization in a variety of different biological samples, ranging from our senses of hearing and balance over breast development and breast cancer, to plant cell walls and microbial communities. In order to accomplish these goals, we continue to adapt and further develop new imaging technologies, including focused ion beam scanning electron microscopy and chemical imaging, including Raman microspectroscopy, and correlative light and electron microscopy. We collaborate with a large number of groups within the US and worldwide and are renowned for faithful sample preparation, including high pressure freezing/freeze substitution and/or cryosectioning, as well as preparation of whole mount frozen-hydrated/vitrified samples. We have a track record in three-dimensional electron microscopy, including cryo-electron tomography as well as sophisticated 3D visualization, segmentation and quantitative analysis.